We extend the analysis of two-hadron fragmentation functions to the subleading twist, discussing also the issue of color gauge invariance. Our results can be used anywhere two unpolarized hadrons are semi-inclusively produced in the same fragmentation region, also at moderate values of the hard scale Q. Here, we consider the example of polarized deep-inelastic production of two hadrons and we give a complete list of cross sections and spin asymmetries up to the subleading twist. Among the results, we highlight the possibility of extracting the transversity distribution with longitudinally polarized targets and also the twist-3 distribution $e\left(x\right),$ which is related to the pion-nucleon $\sigma$ term and to the strangeness content of the nucleon.

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